一株重组鸡传染性支气管炎病毒的分离及结构蛋白基因变异和血清型分析

doi:10.11843/j.issn.0366-6964.2015.05.018

Abstract

Abstract:

In order to further understand the genetic variation of circulating infectious bronchitis virus（IBV），S1，E，M and N genes of an IBV strain（GX-NN130048），which was isolated from chickens with immunoprophylaxis defeat in Guangxi，were amplified by reverse transcriptase polymerase chain reaction（RT-PCR），and then cloned，sequenced，and similarity comparison，phylogenetic tree，recombination and serotype analysis were conducted.The results showed that the gene sequence of cleavage site within S protein of GX-NN130048 was RRSRR.The nucleotide similarities of S1，E，M and N genes with those of other reference strains were 60.8%-90.1%，81.0%-94.2%，86.1%-93.8% and 86.0%-93.3%，respectively.According to the phylogenetic tree analysis，it was clustered into 4/91-type based on S1 gene，while its E，M and N genes belonged to LX4-type.Recombination event was detected in S1 gene，which was a recombinant between vaccine strain 4/91 and isolate GX-HC1006（LX4-type）.The serotype analysis revealed that the serotype of GX-NN130048 was different from vaccine strains H120 and 4/91.The results indicated that isolate GX-NN130048 was a recombinant strain and variation existed both in structural genes and serotype.Our results also provide the evidence that the vaccine strain 4/91 has been increasingly involved in genetic variation of IBV epidemic strains，which may lead to the emergence of new serotype，and that may be one of the main causes of vaccine break in the field.Therefore，it is necessary to strengthen the monitoring of IBV field isolates and development of new vaccines.

CLC Number:

S852.659.6

WU Cui-lan，HE Yi-ning，LI He-ming，SUN Xin-kuan，TAN Yu-cai，WEI Tian-chao，MO Mei-lan，WEI Ping. Isolation and Analysis of Structural Protein Genes Variation and Serotype of a Recombinant Strain of Chicken Infectious Bronchitis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 815-823.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.05.018

https://www.xmsyxb.com/EN/Y2015/V46/I5/815

References

［1］苏敬良，高福，索勋，译.禽病学［M］.12版.北京：中国农业出版社，2012：108-130.
SU J L，GAO F，SUO X，the main translation.Diseases of poultry［M］.12^th edition. Beijing：China Agriculture Press，2012：108-130.（in Chinese）
［2］MO M L，HONG S M，KWON H J，et al.Genetic diversity of spike，3a，3b and E genes of infectious bronchitis viruses and emergence of new recombinants in Korea［J］.Viruses，2013，5（2）：550-567.
［3］CAVANAGH D.Severe acute respiratory syndrome vaccine development：Experiences of vaccination against avian infectious bronchitis coronavirus［J］.Avian Pathol，2003，32（6）：567-582.
［4］SELIM K，ARAFA A S，HUSSEIN A H，et al.Molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in Egypt during 2012［J］.Int J V Sci Med，2013，1（1）：102-108.
［5］孙裴，施雷，魏建忠，等.鸡传染性支气管炎病毒感染的分子机制［J］.动物医学进展，2013，34（12）：199-203.
SUN P，SHI L，WEI J Z，et al.Advance in molecular mechanism of infectious bronchitis virus infection［J］. Progress in Veterinary Medicine，2013，34（12）：199-203.（in Chinese）
［6］LIU X L，SU J L，ZHAO J X，et al.Complete genome sequence analysis of a predominant infectious bronchitis virus（IBV） strain in China［J］.Virus Genes，2009，38（1）：56-65.
［7］肖朝庭，时莉，宋翥远，等.传染性支气管炎病毒疫苗株H52的全基因组克隆与分析［J］.中国动物传染病学报，2009，17（2）：17-23.
XIAO C T，SHI L，SONG Z Y，et al.Cloning and sequence analysis of the complete genome of infectious bronchitis virus vaccine strain H52［J］.Chinese Journal of Animal Infectious Diseases，2009，17（2）：17-23.（in Chinese）
［8］KUO S M，KAO H W，HOU M H，et al.Evolution of infectious bronchitis virus in Taiwan：Positively selected sites in the nucleocapsid protein and their effects on RNA-binding activity［J］.Vet Microbiol，2013，162（2-4）：408-418.
［9］JIA W，KARACA K，PARRISH C R，et al.A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains［J］.Arch Virol，1995，140（2）：259-271.
［10］CAVANAGH D，DAVIS P J，COOK J K，et al.Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus［J］.Avian Pathol，1992，21（1）：33-43.
［11］磨美兰，李孟，韦平，等.鸡传染性支气管炎病毒广西流行株3种主要结构蛋白基因的遗传变异分析［J］.畜牧兽医学报，2010，41（9）：1138-1146.
MO M L，LI M，WEI P，et al.Genetic variations of main structural genes of infectious bronchitis virus strains isolated in Guangxi province of China［J］.Acta Veterinaria et Zootechnica Sinica，2010，41（9）：1138-1146.（in Chinese）
［12］胡北侠，杨少华，许传田，等.2008-2012年山东省鸡传染性支气管炎病毒遗传演化分析和致病性研究［J］.畜牧兽医学报，2014，45（5）：795-801.
HU B X，YANG S H，XU C T，et al.Phylogenetic analysis and pathogenicity infectious bronchitis virus isolated in Shangdong province during 2008-2012［J］.Acta Veterinaria et Zootechnica Sinica，2014，45（5）：795-801.（in Chinese）
［13］磨美兰，李孟，韦正吉，等.广西1985年～2008年IBV分离株S1基因高变区I和N基因的序列和系统进化分析［J］.中国预防兽医学报，2009，31（10）：761-766.
MO M L，LI M，WEI Z J，et al.Sequence and phylogenetic analysis of S1 gene hypervariable region I and N genes of infectious bronchitis viruses isolated in Guangxi province of China from 1985 to 2008［J］.Chinese Journal of Preventive Veterinary Medicine，2009，31（10）：761-766.（in Chinese）
［14］MO M L，HUANG B C，WEI P，et al.Complete genome sequences of two Chinese virulent avian coronavirus infectious bronchitis virus variants［J］.J Virol， 2012，86（19）：10903-10904.
［15］王秀英，韦平，陈秋英，等.鸡传染性支气管炎病毒广西分离株血清型的分析［J］.中国兽医学报，2010，30（2）：183-187.
WANG X Y，WEI P，CHEN Q Y，et al.Serotyping of Guangxi isolates of infectious bronchitis virus［J］.Chinese Journal of Veterinary Science，2010，30（2）：183-187.（in Chinese）
［16］李康然，韦平，梁梅芳，等.用气管环培养中和试验对鸡传染性支气管炎病毒进行血清定型［J］.广西农院学报，1991，10（3）：1-6.
LI K R，WEI P，LIANG M F，et al.The serotyping of Guangxi isolates of avian infectious bronchitis virus by neutralization test in chicken tracheal cultures［J］.Journal of Guangxi Agricultural College，1991，10（3）：1-6.（in Chinese）
［17］YU L，JIANG Y，LOW S，et al.Characterization of three infectious bronchitis virus isolates from China associated with proventriculus in vaccinated chickens［J］.Avian Dis，2001，45（2）：416-424.
［18］HAN Z X，SUN C Y，YAN B，et al.A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China［J］.Infect Genet Evol，2011，11（1）：190-200.
［19］磨美兰，李孟，何坤，等.近期广西鸡传染性支气管炎流行概况及防控措施［J］.广西畜牧兽医，2013，29（2）：67-70.
MO M L，LI M，HE K，et al.The general situation and preventive measure of infectious bronchitis virus in Guangxi province recently［J］.Guangxi Journal of Animal Husbandry & Veterinary Medicine，2013，29（2）：67-70.（in Chinese）
［20］CAVANAGH D.Coronavirus avian infectious bronchitis virus［J］.Vet Res，2007，38（2）：281-297.
［21］POHUANG T，CHANSIRIPORNCHAI N，TAWATSIN A，et al.Sequence analysis of S1 genes of infectious bronchitis virus isolated in Thailand during 2008-2009：Identification of natural recombination in the field isolates［J］.Virus Genes，2011，43（2）：254-260.
［22］MARDANI K，NOORMOHAMMADI A H，IGNJATOVIC J，et al.Naturally occurring recombination between distant strains of infectious bronchitis virus［J］.Arch Virol，2010，155（10）：1581-1586.
［23］OVCHINNIKOVA E V，BOCHKOV Y A，SHCHERBAKOVA L O，et al.Molecular characterization of infectious bronchitis virus isolates from Russia and neighbouring countries：Identification of intertypic recombination in the S1 gene［J］.Avian Pathol，2011，40（5）：507-514.

［24］ZHANG Y，WANG H N，WANG T，et al.Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120［J］.Virus Genes，2010，41（3）：377-388.
［25］LI M，MO M L，HUANG B C，et al.Continuous evolution of avian infectious bronchitis virus resulting in different variants co-circulating in Southern China［J］.Arch Virol，2013，158（8）：1783-1786.
［26］MO M L，LI M，HUANG B C，et al.Molecular characterization of major structural protein genes of avian coronavirus infectious bronchitis virus isolates in Southern China［J］.Viruses，2013，5（12）：3007-3020.
［27］LIU X L，MA H J，XU Q Q，et al.Characterization of a recombinant coronavirus infectious bronchitis virus with distinct S1 subunits of spike and nucleocapsid genes and a 3’untranslated region［J］.Vet Microbiol，2013，162（2-4）：429-436.
［28］张小荣，程靖华，张建军，等.QX型与4/91型传染性支气管炎病毒毒株间的重组分析［C］.中国畜牧兽医学会禽病学分会第十六次学术研讨会论文集，2012.
ZHANG X R，CHENG J H，ZHANG J J，et al.Recombination analysis of infectious bronchitis virus between QX-type and 4/91-type［C］.Poultry epidemiology branch of Chinese association of animal science and veterinary medicine on the 16^th academic conference proceedings，2012.（in Chinese）
［29］刘胜旺.我国鸡传染性支气管炎流行现状及原因分析［J］.中国家禽，2010，32（16）：5-9.
LIU S W.The current situation and analysis of causes of infectious bronchitis virus in China［J］. China Poultry，2010，32（16）：5-9.（in Chinese）
［30］孙蓉.鸡传染性支气管炎病毒广西分离株血清型的鉴定及免疫原性的研究［D］.南宁：广西大学，2012.
SUN R.Serotype identification and immunogenicity of Guangxi isolates of avian infectious bronchitis virus［D］. Nanning：Guangxi University，2012.（in Chinese）
［31］王秀英，李孟，韦平，等.鸡传染性支气管炎病毒广西分离毒株抗原相关性的研究［J］.病毒学报，2012，28（6）：621-627.
WANG X Y，LI M，WEI P，et al.Evaluation of antigenic relationship of Guangxi isolates of infectious bronchitis virus［J］. Chinese Journal of Virology，2012，28（6）：621-627.（in Chinese）
［32］秦丽莉，李孟，孙蓉，等.广西2009~2011年鸡传染性支气管炎病毒分离株的基因分型及血清分型［J］.病毒学报，2014，30（2）：162-170.
QIN L L，LI M，SUN R，et al.Genotypes and serotypes of avian infectious bronchitis viruses isolated during 2009-2012 in Guangxi，China［J］.Chinese Journal of Virology，2014，30（2）：162-170.（in Chinese）
［33］THOR S W，HILT D A，KISSINGER J C，et al.Recombination in avian gamma-coronavirus infectious bronchitis virus［J］.Viruses，2011，3（9）：1777-1799.

[1]	SONG Wenyan, ZHANG Hanwen, WU Aodi, ZHANG Liyan, LIU Zhao, YE Tongtong, CHEN Chuangfu, SHENG Jinliang. Screening of Nanobodies against Porcine Reproductive and Respiratory Syndrome Virus GP5 Protein and Exploration of Their Inhibitory Effect on Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 258-270.
[2]	WANG Ying, LI Jiakang, ZENG Yue, SHI Kaituo, WEI Liting, PENG Jiajia, LI Qiuyan, CAO Longlong, ZHOU Dengyuan. Development of Monoclonal Antibodies against Feline Calicivirus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 401-405.
[3]	LI Huihui, DONG Keer, LIU Xinbo, ZHANG Chunxiao, MA Chao, CHEN Liping, ZHONG Qi, YAO Gang, MA Xuelian. Establishment and Application of Rapid Detection Method for Bovine Norovirus and Bovine Rotavirus Dual RAA-LFD [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 406-412.
[4]	ZHOU Jianhao, WANG Dongfang, LIU Ying, WANG Shujuan, MA Zhenyuan, XIE Caihua, ZHAO Xueli, YANG Haibo, FENG Guidan, KANG Taisheng, HU Yufeng, LI Bowen, YAN Ruoqian. Establishment and Preliminary Application of a Quantitative Droplet Digital PCR Assay for Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 413-418.
[5]	ZHANG Jiaqi, JIA Xining, ZHOU Qun, SONG Xin, ZHU Chenxi, LI Gege, ZHANG Bin. Construction of BVDV-1 Virus-like Particles based on E2 Protein and Evaluation of Its Immune Efficacy in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5143-5153.
[6]	ZHOU Likun, DONG Xinyuan, LI Jiahui, CUI Zhiying, ZHAO Shijie, XU Jie, CHEN Jing, ZHANG Yina, XIA Ping’an. Regulatory Role of MID2 on Porcine Reproductive and Respiratory Syndrome Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4735-4744.
[7]	ZHENG Ruwen, HUANG Tao, WU Daoyi, YU Guangmei, LI Fangfang, SUI Xin, MIN Tingyu. Establishment and Preliminary Application of LAMP-LFD Detection Method for Bovine Viral Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4745-4753.
[8]	JIA Yiquan, YU Mengting, LIU Weirong, FANG Shouguo, ZHANG Songbai. Baicalin Inhibits Infectious Bronchitis Virus Replication via Promoting the Expression of Type Ⅰ Interferon [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4839-4850.
[9]	CHEN Lu, CHANG Xinyu, SHEN Jiachen, SHEN Ruiting, ZHAO Zhenhua, HOU Xiaolin. Transcriptome Sequencing and Analysis of HD11 Cells Infected by Avian Infectious Bronchitis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4860-4865.
[10]	HE Yeqing, ZUO Baijiao, LI Qinghao, SUN Juan, JIN Xin, XIA Lu, WEI Zhanyong. Histopathological Observation of Immune Organs of Piglets Infected with Transmissible Gastroenteritis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4872-4879.
[11]	CHEN Wenzhe, ZHANG Xiangle, GU Fengxing, ZHAO Zhenxiang, LI Kangli, XUE Zhaoning, ZHENG Haixue, ZHANG Xiaoli, ZHU Zixiang. Evaluation of the Antiviral Effect of Porcine GRK2 against Foot-and-mouth Disease Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4350-4361.
[12]	ZOU Hong, XIA Yingju, LI Ling, XU Lu, ZHAO Junjie, WANG Tuanjie, ZHANG Qianyi, SONG Zhenhui. Establishment and Preliminary Application of a Dual Real-time RT-PCR Assay for CSFV and BVDV [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4422-4427.
[13]	YUAN Li, SUN Yangyang, ZHANG Lujie, ZHANG Jie, SUN Haifeng, BAI Juan, JIANG Ping. Production and Epitope Characterization of Monoclonal Antibodies against GP3 Protein of Porcine Reproductive and Respiratory Syndrome Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3424-3434.
[14]	CAO Liyan, KONG Xiangyu, LI Xiangtong, SUO Xuepeng, DUAN Yueyue, YUAN Cong, SHI Lei, ZHANG Yu, MA Guoxiang, ZHENG Haixue, WANG Qi. Preparation and Sequence Analysis of Monoclonal Antibody against the Nucleocapsid Protein of Porcine Acute Diarrhea Syndrome Coronavirus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2487-2497.
[15]	ZHANG Senhao, WANG Xueying, CAI Limeng, XIE Weichun, KUANG Hongdi, WANG Xiaona, LI Jiaxuan, CUI Wen, JIANG Yanping, ZHOU Han, SHAN Zhifu, WANG Li, QIAO Xinyuan, LI Yijing, TANG Lijie. Establishment and Application of Rapid Diagnosis Method for RPA of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2498-2508.

Isolation and Analysis of Structural Protein Genes Variation and Serotype of a Recombinant Strain of Chicken Infectious Bronchitis Virus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments